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Image Search Results
Journal: Cell stem cell
Article Title: Dppa2/4 facilitate epigenetic remodeling during reprogramming to pluripotency
doi: 10.1016/j.stem.2018.08.001
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Cell Analysis, Recombinant, SYBR Green Assay, Staining, Reverse Transcription, Protease Inhibitor, Magnetic Beads, RNA Amplification, Purification, Silver Staining, Isolation, Mouse Assay, Software
Journal:
Article Title: De-Convoluting the "Omics" for Organ Transplantation
doi: 10.1097/MOT.0b013e32833068fb
Figure Lengend Snippet: Some of the available informatic tools that can be applied towards OMIC analyses
Article Snippet: Genomic Research) X-Miner (X-Mine) R: Statistics for Microarray Analysis (Terry Speed, UC Berkeley) Xpression NTI (InforMax) SAM: Significance Analysis of Microarrays (Tibshirani, Stanford) SNOMAD (Jonathan Pevsner,
Techniques: Microarray, Imaging, Expressing
Journal: Cell Death Discovery
Article Title: CAMK2D: a novel molecular target for BAP1 -deficient malignant mesothelioma
doi: 10.1038/s41420-023-01552-5
Figure Lengend Snippet: a Generation of BAP1 ‐KO cell lines using the CRISPR/Cas9 system using a single guide RNA sequence against exon 4 of the BAP1 gene locus. b Establishment of BAP1 -KO cell clones using human mesothelial cell lines, MeT‐5A and HOMC-D4. BAP1 protein expression was determined by Western blot analysis using GAPDH as the internal control. c Gene expression analysis represented by heatmap of upregulated (seven genes; fold change, >3) and downregulated (40 genes, fold change, <0.1) genes in BAP1 ‐KO cells (#1 and #2) compared with parental (P) and BAP1 -WT (Ctrl) cells. The heatmap was constructed based on the normalized values of all samples using TreeView (Cluster 3.0) http://jtreeview ( http://jtreeview.sourceforge.net ). The corresponding upregulated or downregulated genes in the heatmap are shown on the right side. d RT-PCR analysis for mRNA expression levels of upregulated or downregulated genes in the MeT-5A and HOMC-D4 cells. Representative agarose gels for the RT-PCR products from the parental cells (P), BAP1 -WT cells (Ctrl), and BAP1 -KO cell lines (#1 and #2) are shown.
Article Snippet: The heatmap was constructed based on the normalized values of all samples using
Techniques: CRISPR, Sequencing, Clone Assay, Expressing, Western Blot, Control, Gene Expression, Construct, Reverse Transcription Polymerase Chain Reaction